The project included eight project elements or tasks:
- Nutrients and major ions in transport
- Molecular tracers
- Macroinvertebrate community structure
- Organic particles (suspended solids) in transport
- Dissolved organic carbon (DOC) and biodegradable dissolved organic carbon (BDOC) dynamics
- Nitrogen (N), phosphorous (P), and DOC Spiraling
- Net stream metabolism
- Reservoir primary productivity
Nutrients and Major Ions in Transport
Nutients and major ions in transport were monitored during summer baseflow and during stormflows. Major ions include cations (i.e., sodium, magnesium, calcium, and potassium), and anions (i.e., sulfate, chloride). Nutrients include various forms of nitrogen and phosphorus. pH, alkalinity, and conductivity are also measured at the study sites.
» For a complete description of all nutrient/ion methodologies, data analyses, results, literature cited, and interpretations please see Chapter 3 in the final report.
Molecular tracers are a broad group of compounds present in the aquatic environment that are unique to various contaminant sources. The use of such tracers is an emerging technique that qualitatively links the presence of a particular contaminant in a stream or river to a specific source of that contamination within the upstream watershed. Tracers are measured during summer and winter baseflow and during stormflows and include: fragrances found in domestic products (e.g.,detergents) and caffeine, which are used to indicate the presence of waste water treatment plant (WWTP) or septic effluent; fecal steroids, which track animal (farm or wildlife) and human contamination; and polycyclic aromatic hydrocarbons (PAH), which target urban/suburban sources of contamination.
» For a complete description of all tracer methodologies, data analyses, results, literature cited, and interpretations please see Chapter 4 in the final report.
Macroinvertebrate Community Structure
Macroinvertebrate community structure provides an integrated (over time) measure of water quality conditions. Stream dwelling invertebrates were collected at the 60 stream sites during spring baseflow. Benthic macroinvertebrates, such as insects, worms, and molluscs, provide an extended temporal perspective (relative to the molecular tracer, major ions and nutrient sampling performed periodically) because they have limited mobility and relatively long life spans . Macroinvertebrates have measurable responses to a wide variety of environmental changes and stresses that can be easily analyzed, and they are an important link in the aquatic food web.
» For a complete description of all macroinvertebrate methodologies, data analyses, results, literature cited, and interpretations please see Chapter 7 in the final report.
Organic Particles (Suspended Solids) in Transport
Organic particles in transport are indicative of the ability of a stream ecosystem to process organic matter, provide a link between the upstream generation and downstream transfer of organic energy, and provide an estimate of the carbon loading to downstream reservoirs. The concentration, size distribution, and transport of organic particles were sampled under baseflow conditions and, to estimate response of organic particle transport to runoff events, during stormflow.
» For a complete description of all organic matter transport methodologies, data analyses, results, literature cited, and interpretations please see Chapter 6 in the final report.
DOC and BDOC Dynamics
Dissolved organic carbon (DOC) is an indicator of organic loadings to streams, as well as terrestrial processing (e.g., within the soil, forests, and wetlands) of organic matter. In the absence of extensive wetlands, bogs, or swamps, baseflow concentrations of DOC in undisturbed watersheds generally range from approximately 1 to 3 mg carbon/L. Higher concentrations suggest sources of organic pollution such as point sources from sewage treatment plant discharges or non-point-source runoff from urban or rural landscapes.
The biodegradable DOC fraction (BDOC) consists of organic molecules that heterotrophic bacteria can utilize as a source of energy and carbon. Within the context of drinking water quality, some subset of DOC constitutes the precursors of disinfection byproducts, and the BDOC constitutes the nutritional resources that can contribute to biological regrowth within water distribution systems.
» For a complete description of all organic matter methodologies, data analyses, results, literature cited, and interpretations please see Chapter 6 in the final report.
Nitrogen (N), Phosphorous (P), and Dissolved Organic Carbon (DOC) Spiraling
Phosphorus, nitrogen, and carbohydrates tend to be taken up and recycled several times as they move downstream. This cycling and the simultaneous downstream transport are sometimes referred to as “spiraling.” The “spiraling length” represents the distance over which the average nutrient atom travels as it completes one cycle of utilization from a dissolved available form, passes through one or more metabolic transformations and is returned to a dissolved available form.
Spiraling in the stream ecosystem reflects the degree of metabolic activity within the system, the ability of the system to retain nutrients, and the relative utilization rates (hence degree of nutrient limitation) among different nutrients. Spiraling length also describes the scale on which upstream processes are linked to downstream processes. Thus spiraling represents a fundamental measure of stream ecosystem function.
Ecosystem impairment is likely to increase spiraling length (reduce the cycling intensity) through reduced uptake, excessive loading, or decreased retentive ability of the ecosystem. An exception to this rule would occur when the increased loading of a single nutrient stimulates uptake of a second nutrient, whose spiraling length would shorten. Spiraling lengths of nitrogen, phosphorus and carbon (DOC) are estimated in the ten integrative stream sites under baseflow conditions.
» For a complete description of all N, P, and DOC spiraling methodologies, data analyses, results, literature cited, and interpretations please see Chapter 8 in the final report.
Net Stream Metabolism
Stream metabolism was assessed concurrently with spiraling work (Task 6) at 10 integrative stream sites. Stream metabolism measurements provide data on two fundamental ecosystem functions – primary productivity and community respiration.
Gross primary productivity (GPP) is a measure of the rate of synthesis of plant (primarily algal) biomass, and respiration is an index of the breakdown of reduced chemical energy, including the metabolic costs of photosynthesis. These functional attributes are expected to relate principally to biomass of algae, heterotrophic microorganisms, and, to a lesser extent, macroinvertebrates. The actual rates are also influenced by environmental variables of light, temperature, and dissolved and particulate nutrients.
Changes in activity, or in the balance of activity, over time would be an important signal that watershed activities are affecting function in a stream entering a reservoir, and would indicate a need for follow-up work on upstream tributaries.
» For a complete description of all stream metabolism methodologies, data analyses, results, literature cited, and interpretations please see Chapter 9 in the final report.
Reservoir Primary Productivity
Measuring primary productivity in reservoirs allowed us to determine whether there is a link between the reservoirs and the primary tributaries to these reservoirs. If the major tributary to a reservoir contributes the most nutrients to the reservoir, we would expect that reservoirs would rank in the same order as tributaries with respect to metabolic activity. If they do not rank similarly, and the pattern was sustained over a period of years, we would infer that nutrients from other sources – or reservoir morphology or other physical characteristics – are more important regulators of metabolic activity than nutrients from the primary tributary. Just as for the influent streams, a change in activity over time will suggest that watershed changes are affecting processes in the system.
» For a complete description of all primary productivity methodologies, data analyses, results, literature cited, and interpretations please see Chapter 10 in the final report.