Isolation and analysis of lignin-derived phenols in aquatic humic substances: improvements on the procedures

350 210 Stroud Water Research Center

Standley, L.J., and L.A. Kaplan. 1998. Organic Geochemistry 28(11):689–697.

doi: 10.1016/S0146-6380(98)00041-2


Procedures for the extraction of aquatic humic substances and analysis of their lignin-derived phenols were assessed and altered to simplify and shorten XAD-8 resin cleaning, reduce losses due to sorption, and optimize oxidative release of phenols without increasing their transformation. The revised cleaning procedures require 5 rather than 120 h and sorptive losses of humic acids were reduced 7%. Coefficients of variation for individual phenols in the final method ranged from 1 to 11%. The method was used to analyze lignin-derived phenols in White Clay Creek watershed humics, Suwannee River humics (IHSS), and Lake Washington sediments.