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Adenylate energy charge in streambed sediments

350 210 Stroud Water Research Center

Kaplan, L.A., and T.L. Bott. 1985. Freshwater Biology 15:133–138.

doi: 10.1111/j.1365-2427.1985.tb00187.x

Summary

  1. The adenylate energy charge (ECA) of microbial communities from streambed sediments was measured during three different seasons, under experimental manipulation and in culture.
  2. The ECA values of sediments (x±S.E.) in the autumn, winter and spring were low and constant; 0.22±0.03 (n=12), 0.32±0.04 (n= 12) and 0.28±0.03 (n=6) respectively.
  3. A 5 h exposure of sediments to an algal lysate at 3.0–4.0°C and a 48 h exposure of sediments to tryptone-yeast extract at 8.0–18.0°C failed to increase ECA even though respiration increased 3.7-fold during the latter exposure.
  4. The cellular ECA of a bacterial monoculture, sampled in log phase, was 0.90±0.10 (n=3), but extracellular AMP depressed the total culture ECA to 0.21±0.01 (n=4).
  5. Attempts to isolate extracellular AMP from the interstitial waters of sediments were unsuccessful.
  6. The data suggest that under natural conditions ECA is of limited use as a monitor of subtle changes in the physiological state of microbial communities in streambed sediments.

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