Improved performance of the PacBio SMRT technology for 16S rDNA sequencing

350 210 Stroud Water Research Center

Mosher, J.J., B. Bowman, E.L. Bernberg, O. Shevchenko, J. Kan, J. Korlach, and L.A. Kaplan. 2014. Journal of Microbiological Methods 104:59–60.

doi: 10.1016/j.mimet.2014.06.012


Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of > 1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples.